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pegfp c1 ataxin3 q84 addgene  (Addgene inc)


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    Structured Review

    Addgene inc pegfp c1 ataxin3 q84 addgene
    Pegfp C1 Ataxin3 Q84 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+ataxin3+q84/pEGFP-ATXN1-52Q+(Plasmid+%2332492)/pm37984409-317-36-37
    Average 91 stars, based on 1 article reviews
    pegfp c1 ataxin3 q84 addgene - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Luciferase:

    Article Title: Lanosterol elevates cytoprotective response through induced-proteasomal degradation of aberrant proteins.
    Article Snippet: Efficient protein synthesis is a basic requirement of our cells to replace the old or defective proteins from the intrinsic crowded biomolecular environment.. The interconnection among synthesis, folding, and degradation of proteins represents central paradigm to proteostasis.. Failure of protein quality control (PQC) mechanisms results in the disturbance and inadequate functions of proteome.

    Transfection:

    Article Title: A multi‐layered network model identifies Akt1 as a common modulator of neurodegeneration
    Article Snippet: HEK293 cells for viability assays were cultured in plating medium (Dulbecco's modified Eagle's medium (DMEM, Welgene, South Korea) with 10% fetal bovine serum (FBS, Welgene, South Korea) and 50 μg/ml gentamycin (Duchefa, Netherlands) in a 5% CO 2 humidified atmosphere at 37°C. .. HEK293 cells having 70–80% cell density were transiently transfected with pEGFP‐C1‐Aβ 1‐42 , pEGFP‐Htt‐exon1‐Q74 (Addgene #40262), pEGFP‐Ataxin1‐52Q (Addgene #32492), or pEGFP‐C1‐Ataxin3‐Q84 (Addgene #22123) DNA using Lipofectamine 2000 (Invitrogen, CA, USA) following the manufacturer's instructions. .. The pEGFP‐C1‐Aβ 1–42 plasmid was constructed from pCAX‐FLAG‐APP (Addgene #30154).

    Expressing:

    Article Title: Praja1 RING ‐finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease‐associated protein aggregation
    Article Snippet: .. For the construction of adenovirus vectors expressing 3′‐EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 5′‐DsRed‐ and 3′‐FLAG‐tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α‐synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin‐3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT‐PCR) or from Addgene plasmids pEGFP‐C1‐Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP‐C1‐Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP‐Q23 (Cat. No. #40261; Addgene) and pEGFP‐Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table were cloned into pEGFPN1‐3 and DsRed‐Monomer‐C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described , , , (Fig. ). .. Point mutations of SOD1 (G93A:g281c) and α‐synuclein (A53T:g157a) were created by QuikChange II Site‐Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.
    Article Snippet: .. For the construction of adenovirus vectors expressing 30-EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 50-DsRed- and 30- FLAG-tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α-synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin-3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT-PCR) or from Addgene plasmids pEGFP-C1-Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP-C1-Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP-Q23 (Cat. No. #40261; Addgene) and pEGFP-Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table 1 were cloned into pEGFPN1-3 and DsRed-Monomer-C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described14–16,22 (Fig. 1A, B). .. Point mutations of SOD1 (G93A:g281c) and α-synuclein (A53T:g157a) were created by QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Reverse Transcription:

    Article Title: Praja1 RING ‐finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease‐associated protein aggregation
    Article Snippet: .. For the construction of adenovirus vectors expressing 3′‐EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 5′‐DsRed‐ and 3′‐FLAG‐tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α‐synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin‐3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT‐PCR) or from Addgene plasmids pEGFP‐C1‐Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP‐C1‐Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP‐Q23 (Cat. No. #40261; Addgene) and pEGFP‐Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table were cloned into pEGFPN1‐3 and DsRed‐Monomer‐C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described , , , (Fig. ). .. Point mutations of SOD1 (G93A:g281c) and α‐synuclein (A53T:g157a) were created by QuikChange II Site‐Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.
    Article Snippet: .. For the construction of adenovirus vectors expressing 30-EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 50-DsRed- and 30- FLAG-tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α-synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin-3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT-PCR) or from Addgene plasmids pEGFP-C1-Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP-C1-Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP-Q23 (Cat. No. #40261; Addgene) and pEGFP-Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table 1 were cloned into pEGFPN1-3 and DsRed-Monomer-C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described14–16,22 (Fig. 1A, B). .. Point mutations of SOD1 (G93A:g281c) and α-synuclein (A53T:g157a) were created by QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Praja1 RING ‐finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease‐associated protein aggregation
    Article Snippet: .. For the construction of adenovirus vectors expressing 3′‐EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 5′‐DsRed‐ and 3′‐FLAG‐tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α‐synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin‐3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT‐PCR) or from Addgene plasmids pEGFP‐C1‐Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP‐C1‐Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP‐Q23 (Cat. No. #40261; Addgene) and pEGFP‐Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table were cloned into pEGFPN1‐3 and DsRed‐Monomer‐C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described , , , (Fig. ). .. Point mutations of SOD1 (G93A:g281c) and α‐synuclein (A53T:g157a) were created by QuikChange II Site‐Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.
    Article Snippet: .. For the construction of adenovirus vectors expressing 30-EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 50-DsRed- and 30- FLAG-tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α-synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin-3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT-PCR) or from Addgene plasmids pEGFP-C1-Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP-C1-Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP-Q23 (Cat. No. #40261; Addgene) and pEGFP-Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table 1 were cloned into pEGFPN1-3 and DsRed-Monomer-C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described14–16,22 (Fig. 1A, B). .. Point mutations of SOD1 (G93A:g281c) and α-synuclein (A53T:g157a) were created by QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Clone Assay:

    Article Title: Praja1 RING ‐finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease‐associated protein aggregation
    Article Snippet: .. For the construction of adenovirus vectors expressing 3′‐EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 5′‐DsRed‐ and 3′‐FLAG‐tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α‐synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin‐3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT‐PCR) or from Addgene plasmids pEGFP‐C1‐Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP‐C1‐Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP‐Q23 (Cat. No. #40261; Addgene) and pEGFP‐Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table were cloned into pEGFPN1‐3 and DsRed‐Monomer‐C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described , , , (Fig. ). .. Point mutations of SOD1 (G93A:g281c) and α‐synuclein (A53T:g157a) were created by QuikChange II Site‐Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.
    Article Snippet: .. For the construction of adenovirus vectors expressing 30-EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 50-DsRed- and 30- FLAG-tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α-synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin-3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT-PCR) or from Addgene plasmids pEGFP-C1-Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP-C1-Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP-Q23 (Cat. No. #40261; Addgene) and pEGFP-Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table 1 were cloned into pEGFPN1-3 and DsRed-Monomer-C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described14–16,22 (Fig. 1A, B). .. Point mutations of SOD1 (G93A:g281c) and α-synuclein (A53T:g157a) were created by QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Cloning:

    Article Title: Praja1 RING ‐finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease‐associated protein aggregation
    Article Snippet: .. For the construction of adenovirus vectors expressing 3′‐EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 5′‐DsRed‐ and 3′‐FLAG‐tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α‐synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin‐3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT‐PCR) or from Addgene plasmids pEGFP‐C1‐Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP‐C1‐Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP‐Q23 (Cat. No. #40261; Addgene) and pEGFP‐Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table were cloned into pEGFPN1‐3 and DsRed‐Monomer‐C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described , , , (Fig. ). .. Point mutations of SOD1 (G93A:g281c) and α‐synuclein (A53T:g157a) were created by QuikChange II Site‐Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.
    Article Snippet: .. For the construction of adenovirus vectors expressing 30-EGFP tagged WT Parkin (AxhPRKNEGFP), RNF112/ZNF179 (AxhRNF112EGFP) and RNF220 (AxhRNF220EGFP), and 50-DsRed- and 30- FLAG-tagged WT and P525L FUS (AxDsRhFUSWTFL, AxDsRhFUSP525LFL), WT and G93A SOD1 (AxDsRhSOD1WTFL, AxDsRhSOD1G93AFL), WT and A53T α-synuclein (AxDsRhSNCAWTFL, AxDsRhSNCAA53TFL), ataxin-3 Q28 and Q84 (AxDsRhATXN3Q28FL, AxDsRATXN3Q84FL), and Htt exon 1 Q23 and Q74 (AxDsRhHttEx1Q23FL, AxDsRhHttEx1Q74FL), cDNAs obtained from HEK 293 cells by reverse transcription polymerase chain reaction (RT-PCR) or from Addgene plasmids pEGFP-C1-Ataxin3 Q28 (Cat. No. #22122; Addgene, Watertown, MA, USA), pEGFP-C1-Ataxin3 Q84 (Cat. No. #22123; Addgene), pEGFP-Q23 (Cat. No. #40261; Addgene) and pEGFP-Q74 (Cat. No. #40262; Addgene) by PCR using primers as listed in Table 1 were cloned into pEGFPN1-3 and DsRed-Monomer-C1 (Clontech, Palo Alto, CA, USA), respectively, and SwaI cloning site of adenovirus cassette cosmid pAxCAwtit2 (Cat. No. #6170; TaKaRa, Osaka, Japan) as described14–16,22 (Fig. 1A, B). .. Point mutations of SOD1 (G93A:g281c) and α-synuclein (A53T:g157a) were created by QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA).



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